5E8D

Crystal structure of human epiregulin in complex with the Fab fragment of murine monoclonal antibody 9E5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-22
Detector	RAYONIX MX225HE
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	68.602, 100.291, 187.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.908 - 2.500
R-factor	0.1934
Rwork	0.190
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.329
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	22299
<I/σ(I)>	28.9
Completeness [%]	97.3
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	2-(N-morphilino)ethansulfonic acid monohydrate, PEG4000