5E4E

Engineered Interleukin-13 bound to receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	62.590, 69.370, 186.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.740 - 3.000
R-factor	0.2366
Rwork	0.234
R-free	0.28850
RMSD bond length	0.002
RMSD bond angle	0.526
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.740
High resolution limit [Å]	3.000
Number of reflections	16583
<I/σ(I)>	15.6
Completeness [%]	99.2
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.2	293	lithium sulfate, phosphate/citrate pH 4.2, PEG 1000