Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 176.030, 176.030, 108.071 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.974 - 2.700 |
| R-factor | 0.2361 |
| Rwork | 0.234 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TMI |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.629 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 8.540 | 2.700 |
| Rmerge | 0.240 | 0.066 | 2.840 |
| Rpim | 0.039 | 0.011 | 0.480 |
| Number of reflections | 27622 | ||
| <I/σ(I)> | 16.9 | 57.5 | 1.6 |
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 38.8 | 35.4 | 35 |
| CC(1/2) | 0.999 | 0.999 | 0.415 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% w/v PEG3350, 0.25 M sodium thiocyanate, 100 mM MOPS, pH 7.8, 3% v/v 2,2,2-trifluoroethanol |
