5E2Q
Structure of human DPP3 in complex with angiotensin-II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.953730 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.131, 105.922, 64.846 |
| Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
| Resolution | 45.330 - 2.404 |
| R-factor | 0.2083 |
| Rwork | 0.206 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t6b |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.577 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.330 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.577 | |
| Number of reflections | 30464 | |
| <I/σ(I)> | 9.88 | 1.56 |
| Completeness [%] | 97.4 | 84.18 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.056 M sodium phosphate monobasic monohydrate, 1.344 M potassium phosphate dibasic monohydrate |
