5DSY
Crystal structure of constitutively active PARP-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.12 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.163, 119.900, 120.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.540 - 2.700 |
R-factor | 0.1954 |
Rwork | 0.193 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kjd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.349 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.680 |
Number of reflections | 70646 | |
<I/σ(I)> | 11 | 1.3 |
Completeness [%] | 98.4 | 87.2 |
Redundancy | 12 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2.55-2.65 M NaCl and 0.1 M Tris |