5D71

Crystal structure of MOR04302, a neutralizing anti-human GM-CSF antibody Fab fragment in complex with human GM-CSF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-01-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	158.680, 158.680, 158.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	112.200 - 2.250
R-factor	0.18132
Rwork	0.180
R-free	0.21898
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gmf
RMSD bond length	0.011
RMSD bond angle	1.314
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.200	2.390
High resolution limit [Å]	2.250	2.250
Rmerge	0.074	0.660
Number of reflections	31568
<I/σ(I)>	30.33
Completeness [%]	99.9	99.3
Redundancy	24.9	24.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	100 mM sodium acetate, pH 4.0-4.3, 39-42 % (w/v) PEG 400, 35 mg/mL protein