5D12
Kinase domain of cSrc in complex with RL40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0039 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.270, 63.790, 74.970 |
| Unit cell angles | 101.56, 89.91, 89.98 |
Refinement procedure
| Resolution | 43.490 - 3.000 |
| R-factor | 0.29417 |
| Rwork | 0.290 |
| R-free | 0.37648 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.980 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.100 | |
| High resolution limit [Å] | 3.000 | 16.000 | 3.000 |
| Rmerge | 0.190 | 0.036 | 0.500 |
| Rmeas | 0.231 | 0.045 | 0.600 |
| Total number of observations | 44960 | ||
| Number of reflections | 14548 | 94 | 1350 |
| <I/σ(I)> | 9.39 | 23.18 | 4.24 |
| Completeness [%] | 94.6 | 91.3 | 95.8 |
| Redundancy | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.085-0.125 mM MES, 9-11.5 % PEG20000 |
