5CKX

Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.939
Spacegroup name	P 32 2 1
Unit cell lengths	203.397, 203.397, 67.049
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.716 - 2.700
R-factor	0.202
Rwork	0.200
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w1a
RMSD bond length	0.007
RMSD bond angle	0.675
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.720	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	43154
<I/σ(I)>	8.2
Completeness [%]	98.5
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	0.1 M Tris-HCl, pH 7.9, 0.9 M ammonium sulfate and 10% PEG400