5CCH
Structure of the Ca2+-bound synaptotagmin-1 SNARE complex (short unit cell form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 69.073, 171.626, 146.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
Rmerge | 0.067 | 0.048 | 0.741 |
Rmeas | 0.105 | 0.050 | 0.948 |
Rpim | 0.027 | 0.013 | 0.252 |
Total number of observations | 302949 | ||
Number of reflections | 20922 | ||
<I/σ(I)> | 4 | ||
Completeness [%] | 99.7 | 99.3 | 98.7 |
Redundancy | 14.5 | 14.1 | 12.4 |
CC(1/2) | 0.998 | 0.895 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.25% v/v PEG8000, 25 mM HEPES-Na, 75 mM NaCl, 25 mM MgCl2, 0.25 mM CaCl2 |