5BXV
eIF4E complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2012-03-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.607, 67.830, 79.084 |
Unit cell angles | 90.00, 112.43, 90.00 |
Refinement procedure
Resolution | 38.600 - 2.100 |
R-factor | 0.1634 |
Rwork | 0.162 |
R-free | 0.19480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wkw |
RMSD bond length | 0.012 |
RMSD bond angle | 1.303 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.480 |
Number of reflections | 37630 | |
<I/σ(I)> | 21.8 | 2 |
Completeness [%] | 96.9 | 92.4 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 298 | 0.1 M Tris-HCl, and 22 % polyethylene glycol 4000 |