5BQE

Crystal structure of Norrin in complex with the cysteine-rich domain of Frizzled 4 -Methylated form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 43 2 2
Unit cell lengths	98.920, 98.920, 120.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.460 - 2.300
R-factor	0.1978
Rwork	0.197
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bpb 5bpu
RMSD bond length	0.004
RMSD bond angle	0.927
Data reduction software	MOSFLM
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.460	49.460	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.103	0.034	1.212
Rpim	0.045	0.016	0.561
Total number of observations	161410	2773	14037
Number of reflections	26816
<I/σ(I)>	10.7	30.6	1.4
Completeness [%]	98.9	98.5	97.2
Redundancy	6	5.1	5.6
CC(1/2)	0.998	0.999	0.288

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M Bicine, pH 9.0, 10% PEG6000