5AM8
Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with amyloid-beta 4-10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 73.449, 101.760, 114.361 |
Unit cell angles | 85.23, 86.07, 81.45 |
Refinement procedure
Resolution | 113.780 - 1.900 |
R-factor | 0.18735 |
Rwork | 0.185 |
R-free | 0.22382 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nxq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.342 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 113.800 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.440 |
Number of reflections | 293455 | |
<I/σ(I)> | 5.8 | 1.4 |
Completeness [%] | 93.0 | 84 |
Redundancy | 1.6 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.06 M DIVALENT CATIONS, 0.1 M TRIS/BICINE PH 8.5, 30 % PEG550MME/PEG20000 |