5AM8
Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with amyloid-beta 4-10
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | ANGIOTENSIN-CONVERTING ENZYME | polymer | 629 | 72592.5 | 4 | UniProt (P12821) Pfam (PF01401) | HOMO SAPIENS (HUMAN) | ACE, DIPEPTIDYL CARBOXYPEPTIDASE I, KININASE II, CD143, ANGIOTENSIN-CONVERTING ENZYME, SOLUBLE FORM |
| 2 | E, F, G, H (P, Q, R, S) | BETA-AMYLOID PROTEIN 42 | polymer | 7 | 882.9 | 4 | UniProt (P05067) | HOMO SAPIENS | BETA-APP42, AMYLOID-BETA |
| 3 | I, J, L, O (E, F, H, K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 4 | K, N (G, J) | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 2 | In PDB GlyTouCan (G86851RC) | |||
| 5 | M (I) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 6 | P (L) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 732.7 | 1 | In PDB GlyTouCan (G86566TX) | |||
| 7 | Q (M) | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 732.7 | 1 | In PDB GlyTouCan (G47477HI) | |||
| 8 | AA, JA, QA, R (B, C, D, A) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 9 | BA, KA, RA, S (B, C, D, A) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 10 | CA, LA, SA, T (B, C, D, A) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 11 | TA, U, UA (D, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
| 12 | AB, DA, EA, FA, HA... (D, B, C, A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 18 | Chemie (PEG) | |||
| 13 | GA, WA, X (B, D, A) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 3 | Chemie (P6G) | |||
| 14 | BB, CB, DB, EB, FB... (A, B, C, D, R...) | water | water | 18.0 | 1829 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 629 (UniProt: P12821)
| PDB | External Database | Details |
|---|---|---|
| Gln 9 | Asn 38 | engineered mutation |
| Gln 25 | Asn 54 | engineered mutation |
| Gln 82 | Asn 111 | engineered mutation |
| Gln 117 | Asn 146 | engineered mutation |
| Gln 289 | Asn 318 | engineered mutation |
| Arg 545 | Gln 574 | engineered mutation |
| Leu 576 | Pro 605 | engineered mutation |
| Leu 629 | Arg 658 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 293901.6 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 4484.2 | |
| Non-Polymers* | Number of molecules | 36 |
| Total formula weight | 4208.5 | |
| All* | Total formula weight | 302594.3 |
