5AJA
Crystal structure of mandrill SAMHD1 (amino acid residues 1-114) bound to Vpx isolated from mandrill and human DCAF1 (amino acid residues 1058-1396)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 102.021, 102.021, 265.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.820 - 2.649 |
| R-factor | 0.1761 |
| Rwork | 0.173 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cc9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.810 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.080 | 1.450 |
| Number of reflections | 24555 | |
| <I/σ(I)> | 20.7 | 1.63 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 9.4 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.16 M TRISODIUM CITRATE-HCL PH 5.2, 4% PEG 6000 |
