5A2L
Crystal structure of scFv-SM3 in complex with APD-CGalNAc-RP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.080, 35.190, 66.290 |
Unit cell angles | 90.00, 103.01, 90.00 |
Refinement procedure
Resolution | 64.590 - 1.790 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.20447 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sm3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.528 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.590 | 1.880 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.110 | 0.600 |
Number of reflections | 19905 | |
<I/σ(I)> | 7.6 | 2.4 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |