5A0Q
Cryo-EM reveals the conformation of a substrate analogue in the human 20S proteasome core
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
| Number of Images | 960 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Software | Coot,PHENIX,IMAGIC,SPIDER,TIGRIS |
| Symmetry | C2 |
