5ZV2
FGFR-1 in complex with ligand lenvatinib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.214, 57.464, 65.955 |
| Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
| Resolution | 100.650 - 2.860 |
| R-factor | 0.21475 |
| Rwork | 0.213 |
| R-free | 0.26931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uwy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.232 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.650 | 3.110 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Number of reflections | 17573 | |
| <I/σ(I)> | 21.87 | |
| Completeness [%] | 99.1 | 98 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 17.70 % PEG-5000-MME, 0.20 M (NH4)2SO4, 0.10 M Tris pH 7.00, Cryo 25% Ethylenglycole in reservoir solution. |
