5ZMC

Structural Basis for Reactivation of -146C>T Mutant TERT Promoter by cooperative binding of p52 and ETS1/2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.00
Spacegroup name	P 41 2 2
Unit cell lengths	71.414, 71.414, 262.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.410 - 2.990
R-factor	0.26043
Rwork	0.259
R-free	0.28826
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3q 1k78
RMSD bond length	0.008
RMSD bond angle	1.251
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0216)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.410	3.220
High resolution limit [Å]	2.990	2.990
Rmerge	0.060	0.800
Number of reflections	15992	2514
<I/σ(I)>	20.3
Completeness [%]	99.9	100
Redundancy	6.7	7.2
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.1 M HEPES pH 7.0 and 2.0 M Ammonium sulphate