5YVH
Crystal structure of Karyopherin beta2 in complex with FUS(371-526)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2013-02-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 129.425, 156.532, 67.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.355 - 3.150 |
R-factor | 0.2155 |
Rwork | 0.213 |
R-free | 0.26240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fdd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 3.150 |
Rpim | 0.051 | 0.533 |
Number of reflections | 25064 | |
<I/σ(I)> | 5.4 | 1.34 |
Completeness [%] | 99.6 | |
Redundancy | 5.4 | |
CC(1/2) | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.8M Succinic acid pH7.0 |