5Y31
Crystal structure of human LGI1-ADAM22 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.143, 124.324, 164.663 |
Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
Resolution | 48.993 - 7.125 |
R-factor | 0.2676 |
Rwork | 0.265 |
R-free | 0.31630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.819 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 7.240 |
High resolution limit [Å] | 7.120 | 7.120 |
Number of reflections | 6092 | |
<I/σ(I)> | 13.3 | 4.6 |
Completeness [%] | 97.9 | 96.3 |
Redundancy | 7.1 | 4.6 |
CC(1/2) | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10 % PEG 8000, 0.1 M zinc acetate, 0.1 M MES-Na (pH 6.0) |