5XU8
Crystal structure of human USP2 in complex with ubiquitin and 6-thioguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-17 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.953, 54.650, 72.687 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.810 |
| R-factor | 0.18151 |
| Rwork | 0.179 |
| R-free | 0.23827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hd5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 33397 | 4726 |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 97.9 | 80.1 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 24.5% PEG3350, 0.2 M MgC12, 0.1 M Bis-Tris |
