5X6V

Crystal structure of human heteroheptameric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.90000
Spacegroup name	P 21 21 21
Unit cell lengths	74.099, 93.476, 125.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.360 - 2.020
R-factor	0.195
Rwork	0.193
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x6u 4arz
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	125.450	2.060
High resolution limit [Å]	2.020	2.020
Number of reflections	57406
<I/σ(I)>	13.6
Completeness [%]	99.4
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium citrate, ammonium acetate, PEG 4000