5VK2

Structural basis for antibody-mediated neutralization of Lassa virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97969
Spacegroup name	P 61 2 2
Unit cell lengths	152.697, 152.697, 456.738
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.602 - 3.201
R-factor	0.1908
Rwork	0.189
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ine
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	3.300
High resolution limit [Å]	3.200	3.200
Rpim		0.845
Number of reflections	52823	4465
<I/σ(I)>	16.17	1.1
Completeness [%]	99.7	99.5
Redundancy	12.9	12.6
CC(1/2)	0.999	0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	0.1M Tris pH 8, 15-18% PEG 3350 and 0.1M-0.3M magnesium acetate