5V39
Crystal structure of human vitamin D receptor ligand binding domain in complex with a VDRM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | .97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.000, 52.670, 105.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.310 - 2.200 |
R-factor | 0.172 |
Rwork | 0.167 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.310 |
High resolution limit [Å] | 2.200 |
Number of reflections | 13006 |
<I/σ(I)> | 8.56 |
Completeness [%] | 99.9 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 100mM Tris HCl pH 8.5, 25% PEG 3350 and 200mM Ammonium Sulfate |