5UTZ
Human IL-2/Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.010, 145.730, 107.320 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 86.168 - 2.747 |
| R-factor | 0.1941 |
| Rwork | 0.193 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3U1S 4npy 3n9g 4hp0 2b5i |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.679 |
| Data reduction software | XDS (March 1, 2015) |
| Data scaling software | XDS (March 1, 2015) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.910 |
| High resolution limit [Å] | 2.747 | 2.747 |
| Number of reflections | 67865 | 10720 |
| <I/σ(I)> | 8.44 | 1 |
| Completeness [%] | 98.9 | 96.9 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.987 | 0.396 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 100 mM sodium citrate, pH 5.0, 20% PEG6000 |
