5UPK

CDC42 binds PAK4 via an extended GTPase-effector interface - 3 peptide: PAK4cat, PAK4-N45, CDC42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 1 21 1
Unit cell lengths	58.465, 61.621, 85.791
Unit cell angles	90.00, 91.57, 90.00

Refinement procedure

Resolution	50.010 - 2.400
R-factor	0.22023
Rwork	0.218
R-free	0.26364
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fij
RMSD bond length	0.008
RMSD bond angle	1.351
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.163
Rpim		0.912
Number of reflections	24144
<I/σ(I)>	12
Completeness [%]	100.0	100
Redundancy	10.9	10.6
CC(1/2)		0.311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1M HEPES pH 7.5, 22% PEG1,000