5UN5

Frizzled-8 complex with designed surrogate Wnt agonist, crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	41.200, 77.510, 81.430
Unit cell angles	90.00, 92.76, 90.00

Refinement procedure

Resolution	41.152 - 2.994
R-factor	0.2278
Rwork	0.225
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f0a
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	XDS (March 30, 2013)
Data scaling software	XSCALE (2015-08-21)
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.152	3.050
High resolution limit [Å]	2.980	2.980
Number of reflections	10346	679
<I/σ(I)>	11.5	1.08
Completeness [%]	99.1	88.8
Redundancy	4.1	3.6
CC(1/2)	0.997	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	42-49% PEG 400, 0.1 M Tris pH 7.8-8.2, 0.2 M NaCl