5UKM

bovine GRK2 in complex with human Gbetagamma subunits and CCG258208 (14as)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	189.012, 74.150, 123.175
Unit cell angles	90.00, 115.46, 90.00

Refinement procedure

Resolution	30.000 - 3.030
R-factor	0.1997
Rwork	0.197
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v5w
RMSD bond length	0.012
RMSD bond angle	1.563
Data scaling software	XSCALE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.210
High resolution limit [Å]	3.030	8.940	3.030
Rmerge	0.119	0.038	0.932
Rmeas	0.129	0.041	1.007
Total number of observations	207868
Number of reflections	29942	1226	4657
<I/σ(I)>	12.84	41.18	1.98
Completeness [%]	98.5	97.8	96
Redundancy	6.942	6.748	7.055
CC(1/2)	0.998	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM MES, 0.2 M NaCl, 8-10% PEG3350