5TT2

Inactive conformation of engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 42 21 2
Unit cell lengths	120.224, 120.224, 125.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.429 - 2.949
R-factor	0.2028
Rwork	0.200
R-free	0.25420
RMSD bond length	0.003
RMSD bond angle	0.703
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.949
Rmerge	0.151
Number of reflections	19542
<I/σ(I)>	7.4
Completeness [%]	97.7
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	100mM MES pH6.5 20% PEG3350 200mM Na/K tartrate 200mM lithium sulfate