5SVS
Anomalous Mn2+ signal reveals a divalent cation-binding site in the head domain of the ATP-gated human P2X3 ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.7712 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 120.200, 120.200, 236.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.775 - 4.025 |
| R-factor | 0.2313 |
| Rwork | 0.229 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 4.030 |
| Number of reflections | 10534 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 99.7 |
| Redundancy | 11.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 25% PEG 400, 100 mM MES, pH 6.85, 50 mM MnCl2 |
