5OOB

COMPLEX OF HUMAN NUCLEAR CAP-BINDING COMPLEX WITH M7GTP AND NELF-E C-TERMINAL PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.966
Spacegroup name	P 1 21 1
Unit cell lengths	113.800, 147.230, 153.880
Unit cell angles	90.00, 91.48, 90.00

Refinement procedure

Resolution	153.830 - 2.790
R-factor	0.20331
Rwork	0.202
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2t
RMSD bond length	0.009
RMSD bond angle	1.312
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	159.000	2.870
High resolution limit [Å]	2.790	2.790
Number of reflections	123434	8453
<I/σ(I)>	9.2	0.91
Completeness [%]	98.4	91.6
Redundancy	2.71	2.7
CC(1/2)	0.995	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	6 mg/ml of CBC were mixed with 500 microM m7GTP and 500 microM NELF-E peptide in 120 mM NaCl, 1 mM tris(2-carboxyethyl)phosphine, 20 mM HEPES pH 7.8. Crystals grew at 20 C in mother liquor containing 0.2 M lithium citrate and 20% (w/v) PEG 3350.