5OMX

X-ray Structure of the H2A-N38C Nucleosome Core Particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	106.753, 182.578, 109.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.140 - 2.320
Rwork	0.222
R-free	0.25945
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kx5
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.140	2.450
High resolution limit [Å]	2.320	2.320
Rmerge	0.069	0.212
Number of reflections	80720	3876
<I/σ(I)>	10.3	1.4
Completeness [%]	86.7	28.7
Redundancy	3.2	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	8 mg/ml sample was mixed 1:1 with 10 mM K-Cacodylate (pH 6.0), 140-150 mM MnCl2, 100 KCl. and equilibrated against a 1:4 dilution of the same solution