5OL0
Structure of Leishmania infantum Silent Information Regulator 2 related protein 1 (LiSIR2rp1) in complex with acetylated p53 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.020, 65.670, 66.620 |
| Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
| Resolution | 46.560 - 1.990 |
| R-factor | 0.17005 |
| Rwork | 0.167 |
| R-free | 0.22577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3glt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.832 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.560 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 38331 | 5913 |
| <I/σ(I)> | 15.43 | 2.2 |
| Completeness [%] | 98.8 | 95.4 |
| Redundancy | 3.3 | 3.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 100mM Na Hepes pH7.5; 25% PEG2000 MME |
