5NLB

Crystal structure of human CUL3 N-terminal domain bound to KEAP1 BTB and 3-box

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	41.000, 233.380, 164.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.382 - 3.450
R-factor	0.2406
Rwork	0.238
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ap2
RMSD bond length	0.011
RMSD bond angle	1.438
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.382
High resolution limit [Å]	3.450
Number of reflections	10899
<I/σ(I)>	12.94
Completeness [%]	99.9
Redundancy	6.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% PEG3350, 10% ethylene glycol, 0.2M potassium citrate tribasic