5NG6

Crystal structure of FnCas12a bound to a crRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00768
Spacegroup name	P 21 21 2
Unit cell lengths	214.684, 277.061, 135.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.239 - 3.342
R-factor	0.2397
Rwork	0.239
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5id6
RMSD bond length	0.012
RMSD bond angle	0.687
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	169.700	3.540
High resolution limit [Å]	3.340	3.340
Number of reflections	225590	33814
<I/σ(I)>	10.9	1.73
Completeness [%]	99.7	98.1
Redundancy	7	7
CC(1/2)	0.997	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	50 mM HEPES-NaOH, pH 6.5, 20% (w/v) Polyethylene glycol 3,400, 1.5% (w/v) Tryptone