5NFV
Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00768 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.421, 143.703, 84.628 |
| Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
| Resolution | 46.568 - 2.501 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.592 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.568 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 | 0.878 |
| Number of reflections | 131823 | |
| <I/σ(I)> | 14.6 | 1.49 |
| Completeness [%] | 98.5 | 96.5 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.999 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris propane (pH 6.5), 0.2 M KSCN, 20% (w/v) polyethylene glycol 3,350 |
