5LX5
CRYSTAL STRUCTURE OF VISFATIN IN COMPLEX WITH SAR154782-RP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.710, 106.690, 83.460 |
Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
Resolution | 65.510 - 1.880 |
Rwork | 0.163 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER-TNT |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.760 | 1.930 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.102 | 0.452 |
Number of reflections | 84857 | |
<I/σ(I)> | 7.3 | 2.1 |
Completeness [%] | 98.6 | 95.8 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 292 | CHES 100mM - PEG8000 14% - Malonate Na 200mM - pH8.8 |