5JHG
Crystal structure of the complex between the human RhoA and the DH/PH domain of human ARHGEF11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-20 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.541782 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.440, 118.589, 88.003 |
| Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
| Resolution | 31.130 - 2.500 |
| R-factor | 0.22362 |
| Rwork | 0.221 |
| R-free | 0.27022 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.969 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.530 |
| Number of reflections | 56052 | |
| <I/σ(I)> | 15.95 | |
| Completeness [%] | 98.2 | 93.4 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 2% v/v Tacsimate pH 5.0, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 16% PEG 3350 |
