5IGM
Crystal structure of the bromodomain of human BRD9 in complex with bromosporine (BSP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.177, 125.677, 29.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.690 - 1.600 |
| R-factor | 0.22248 |
| Rwork | 0.220 |
| R-free | 0.26581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hme 2grc 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.980 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.029 | 0.503 |
| Number of reflections | 35807 | |
| <I/σ(I)> | 28 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M bis tris pH 5.5, 0.2 M sodium chloride, 25% PEG3350 |
