5IGL
Crystal structure of the second bromodomain of human TAF1L in complex with bromosporine (BSP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 44.172, 89.177, 117.892 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.460 - 2.100 |
| R-factor | 0.21484 |
| Rwork | 0.211 |
| R-free | 0.29418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2grc 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.337 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.036 | 0.369 |
| Number of reflections | 13934 | |
| <I/σ(I)> | 16.6 | 2.7 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 63%(v/v) MPD, 0.1M MMT pH 7.5 |
