5IC1
Structural analysis of a talin triple domain module, E1794Y, E1797Y, Q1801Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6424 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.633, 67.193, 65.955 |
| Unit cell angles | 90.00, 101.48, 90.00 |
Refinement procedure
| Resolution | 48.860 - 2.200 |
| R-factor | 0.21037 |
| Rwork | 0.208 |
| R-free | 0.24893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w8p 4f7g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.900 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 26339 |
| <I/σ(I)> | 17.9 |
| Completeness [%] | 99.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M potassium phosphate, 20% (w/v) PEG 3350 |
