5HY8
Glycation restrains allosteric transition in hemoglobin: The molecular basis of oxidative stress under hyperglycemic conditions in diabetes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.954 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 237.987, 59.267, 137.024 |
| Unit cell angles | 90.00, 125.36, 90.00 |
Refinement procedure
| Resolution | 112.130 - 2.300 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hho |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.948 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.130 | 3.360 |
| High resolution limit [Å] | 2.000 | 2.300 |
| Rmerge | 0.386 | |
| Number of reflections | 69814 | |
| <I/σ(I)> | 23.7 | |
| Completeness [%] | 87.0 | 55.1 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 4000 |
