5HXB

Cereblon in complex with DDB1, CC-885, and GSPT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 2 1
Unit cell lengths	156.747, 111.522, 175.057
Unit cell angles	90.00, 95.81, 90.00

Refinement procedure

Resolution	50.000 - 3.600
R-factor	0.226
Rwork	0.224
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tz4 3e1y
RMSD bond length	0.017
RMSD bond angle	1.796
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Rmerge	0.198	0.796
Number of reflections	66582
<I/σ(I)>	10.6	2.1
Completeness [%]	94.6	93.9
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		280	200mM Sodium Citrate, Tris pH 8.5, 18% PEG 3350