5HCC
Ternary complex of human Complement C5 with Ornithodoros moubata OmCI and Dermacentor andersoni RaCI3.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.867, 140.284, 211.293 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.570 - 2.590 |
| R-factor | 0.2323 |
| Rwork | 0.231 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cu7 2cm9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.606 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.600 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.079 | 0.787 |
| Number of reflections | 97166 | |
| <I/σ(I)> | 9.3 | 1.44 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 294 | 10% PEG 20K, 2% v/v 1,4 Dioxane, 0.1 M Bicine pH 9.0 |
