5GTM

Modified human MxA, nucleotide-free form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-02
Detector	RAYONIX MX-225
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	104.180, 60.160, 153.920
Unit cell angles	90.00, 98.62, 90.00

Refinement procedure

Resolution	39.198 - 2.896
R-factor	0.2066
Rwork	0.204
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p4u 3ljb
RMSD bond length	0.010
RMSD bond angle	1.356
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	3.000
High resolution limit [Å]	2.896	2.900
Rmerge	0.122
Number of reflections	42599
<I/σ(I)>	11.2
Completeness [%]	98.8
Redundancy	3.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100mM ammonium citrate tribasic (pH 7.0), 12% PEG 3350