5FPB
Crystal structure of human KDM4D in complex with 2-1H-pyrazol-4-yloxy- 3H,4H-pyrido-3,4-d-pyrimidin-4-one
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-09 |
| Detector | RIGAKU SATURN A200 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.180, 71.180, 150.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.150 - 1.910 |
| R-factor | 0.16243 |
| Rwork | 0.160 |
| R-free | 0.20319 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.860 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 30844 | |
| <I/σ(I)> | 18.8 | 3.9 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.1M HEPES PH 7.4, 2.45M AMMONIUM SULPHATE |
