5FLV
Crystal structure of NKX2-5 and TBX5 bound to the Nppa promoter region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.080, 158.620, 87.860 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 19.966 - 3.005 |
| R-factor | 0.196 |
| Rwork | 0.190 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x6v |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 1.420 |
| Number of reflections | 31026 | |
| <I/σ(I)> | 0.95 | |
| Completeness [%] | 97.9 | 91.3 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M HEPES PH7.5 0.1 M KBR, 0.2M NA-ACETATE AND 20 % PEG 3000 |
