5FLV

Crystal structure of NKX2-5 and TBX5 bound to the Nppa promoter region

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, E, I, M)	HOMEOBOX PROTEIN NKX-2.5, T-BOX TRANSCRIPTION FACTOR TBX5	polymer	285	32947.8	4	UniProt (P42582) Pfam (PF00046) Pfam (PF00907) UniProt (by SIFTS) (P70326)	MUS MUSCULUS (HOUSE MOUSE)	CARDIAC-SPECIFIC HOMEOBOX, HOMEOBOX PROTEIN CSX, HOMEOBOX PROTEIN NK-2 HOMOLOG E, T-BOX PROTEIN 5
2	B, E, H, K (B, F, J, N)	5'-D(*TP*CP*TP*TP*CP*TP*CP*AP*CP*AP*CP*CP*TP*TP *TP*GP*AP*AP*GP*TP*GP*G)-3'	polymer	22	6693.3	4		MUS MUSCULUS
3	C, F, I, L (C, G, K, O)	5'-D(*AP*CP*CP*AP*CP*TP*TP*CP*AP*AP*AP*GP*GP*TP *GP*TP*GP*AP*GP*AP*AP*G)-3'	polymer	22	6809.4	4		MUS MUSCULUS
4	M, N (A, C)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)

Sequence modifications

A, E, I, M: 134 - 197 (UniProt: P42582)

PDB	External Database	Details
Gly 130	-	expression tag
Ala 131	-	expression tag
Met 132	-	expression tag
Gly 133	-	expression tag
Ser 192	Cys 192	engineered mutation
Ser 198	-	linker
Ser 199	-	linker
Ser 200	-	linker
Ser 201	-	linker
Ser 202	-	linker
Ser 203	-	linker
Ser 204	-	linker
Ser 205	-	linker
Ser 206	-	linker
Ser 207	-	linker
Ser 208	-	linker
Ser 209	-	linker
Ser 210	-	linker
Ser 211	-	linker
Ser 212	-	linker
Ala 213	-	linker

A, E, I, M: 1051 - 1251 (UniProt: P42582)

PDB	External Database	Details
Ser 1202	Cys 202	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	185802.2
Non-Polymers*	Number of molecules	2
	Total formula weight	388.5
All*	Total formula weight	186190.7

*Water molecules are not included.