5F98

Crystal structure of RIG-I in complex with Cap-0 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.116
Spacegroup name	P 21 21 21
Unit cell lengths	111.180, 174.570, 309.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.003 - 3.280
R-factor	0.2222
Rwork	0.221
R-free	0.27740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e3h
RMSD bond length	0.002
RMSD bond angle	0.541
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.003	3.400
High resolution limit [Å]	3.100	3.300
Rmerge	0.560
Number of reflections	92301
<I/σ(I)>	7.1	1.9
Completeness [%]	99.8	99.7
Redundancy	14	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	20% (w/v) PEG 3350, 0.2 M NaSCN, 100 mM MOPS (pH 7.8), 3.5% (v/v) 2,2,2-trifluoroethanol