5F8A
Crystal structure of the ternary EcoRV-DNA-Lu complex with uncleaved DNA substrate. Lanthanide binding to EcoRV-DNA complex inhibits cleavage.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-11-18 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.430, 52.860, 65.310 |
| Unit cell angles | 70.61, 73.24, 81.81 |
Refinement procedure
| Resolution | 36.673 - 1.760 |
| R-factor | 0.16402 |
| Rwork | 0.160 |
| R-free | 0.19914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b95 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.574 |
| Data reduction software | iMOSFLM (7.2.1) |
| Data scaling software | SCALA (CCP4interface 6.5.019) |
| Phasing software | PHASER (CCP4interface 6.5.019) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.673 | 1.850 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.051 | 0.196 |
| Number of reflections | 51697 | |
| <I/σ(I)> | 14.7 | 4.4 |
| Completeness [%] | 93.0 | 88.9 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 285 | 4.5 mg/mL (monomer), protein(dimer):DNA - 1:1, Enzyme:Well - 3uL:3uL, Well - 500uL of 100 mM HEPES pH 7.4, 8% Ethylene glycol, 4% Polyethylene glycol 8000, 10% glycerol. |
